Analysis of the inhibitory effect of canavanine on the replication of influenza RI/5+ virus I. Inhibition of assembly of RNP
Identifieur interne : 002925 ( Main/Exploration ); précédent : 002924; suivant : 002926Analysis of the inhibitory effect of canavanine on the replication of influenza RI/5+ virus I. Inhibition of assembly of RNP
Auteurs : Koichiro Maeno [Japon] ; Saiji Yoshii [Japon] ; Kunji Mita [Japon] ; Michinari Hamaguchi [Japon] ; Tetsuya Yoshida [Japon] ; Masao Iinuma [Japon] ; Yoshiyuki Nagai [Japon] ; Toshisada Matsumoto [Japon]Source :
- Virology [ 0042-6822 ] ; 1979.
English descriptors
- Teeft :
- Actinomycin, Analog, Arginine, Arginine analog, Biological activities, Canavanine, Clone cells, Culture fluids, Cytoplasmic, Cytoplasmic extracts, Escherichia coli, Final concentration, Fowl plague virus, Immunofluorescent staining, Infection, Influenza, Influenza virus, Inhibitory effect, Kilbourne, Linear sucrose density gradients, Maeno, Nuclear extracts, Nuclear fractions, Nucleocapsid protein, Polypeptide, Relative amounts, Replication, Simultaneous addition, Soluble form, Subcellular fractions, Sucrose, Sucrose cushions, Viral, Viral antigens, Virology, Virus, Whole cells.
Abstract
Abstract: When influenza A2/RI/5+ virus-infected 1-5C-4 cells were incubated in medium containing 2 μg/ml of canavanine (an arginine analog) from 4 hr after infection, virus growth was completely inhibited. The mechanisms of inhibition by canavanine were investigated by immunofluorescent staining or isotope labeling of cells. The results indicated that in canavanine-treated cells all known viral proteins were synthesized, but most of the nucleocapsid protein (NP) and nonstructural protein (NS) was present in the nucleus, in contrast to control cells in which they were distributed throughout the whole cells. Further, formation of viral ribonucleoprotein (RNP) was inhibited and most NP was present in a nonassembled, soluble form. This action of canavanine was reversible. When arginine was added to canavanine-treated cells, viral RNP soon became detectable in the cytoplasm, and this was followed by the production of infectious virus. However, simultaneous addition of cycloheximide with arginine did not restore the formation of RNP or virus production. Based upon these findings, it is suggested that the inhibitory effect of canavanine on the replication of influenza A2/RI/5+ virus is inhibition of assembly of viral RNP.
Url:
DOI: 10.1016/0042-6822(79)90443-4
Affiliations:
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cells</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: When influenza A2/RI/5+ virus-infected 1-5C-4 cells were incubated in medium containing 2 μg/ml of canavanine (an arginine analog) from 4 hr after infection, virus growth was completely inhibited. The mechanisms of inhibition by canavanine were investigated by immunofluorescent staining or isotope labeling of cells. The results indicated that in canavanine-treated cells all known viral proteins were synthesized, but most of the nucleocapsid protein (NP) and nonstructural protein (NS) was present in the nucleus, in contrast to control cells in which they were distributed throughout the whole cells. Further, formation of viral ribonucleoprotein (RNP) was inhibited and most NP was present in a nonassembled, soluble form. This action of canavanine was reversible. When arginine was added to canavanine-treated cells, viral RNP soon became detectable in the cytoplasm, and this was followed by the production of infectious virus. However, simultaneous addition of cycloheximide with arginine did not restore the formation of RNP or virus production. Based upon these findings, it is suggested that the inhibitory effect of canavanine on the replication of influenza A2/RI/5+ virus is inhibition of assembly of viral RNP.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Maeno, Koichiro" sort="Maeno, Koichiro" uniqKey="Maeno K" first="Koichiro" last="Maeno">Koichiro Maeno</name></noRegion><name sortKey="Hamaguchi, Michinari" sort="Hamaguchi, Michinari" uniqKey="Hamaguchi M" first="Michinari" last="Hamaguchi">Michinari Hamaguchi</name><name sortKey="Iinuma, Masao" sort="Iinuma, Masao" uniqKey="Iinuma M" first="Masao" last="Iinuma">Masao Iinuma</name><name sortKey="Matsumoto, Toshisada" sort="Matsumoto, Toshisada" uniqKey="Matsumoto T" first="Toshisada" last="Matsumoto">Toshisada Matsumoto</name><name sortKey="Mita, Kunji" sort="Mita, Kunji" uniqKey="Mita K" first="Kunji" last="Mita">Kunji Mita</name><name sortKey="Nagai, Yoshiyuki" sort="Nagai, Yoshiyuki" uniqKey="Nagai Y" first="Yoshiyuki" last="Nagai">Yoshiyuki Nagai</name><name sortKey="Yoshida, Tetsuya" sort="Yoshida, Tetsuya" uniqKey="Yoshida T" first="Tetsuya" last="Yoshida">Tetsuya Yoshida</name><name sortKey="Yoshii, Saiji" sort="Yoshii, Saiji" uniqKey="Yoshii S" first="Saiji" last="Yoshii">Saiji Yoshii</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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